Determination of expression level of <i>AP1S1, CDK9, FIGF</i> and <i>HDAC11</i> genes in bladder tumors for aggressive phenotype characterization

Bladder cancer holds a steady 10th place among oncological diseases. Follow-up and timely diagnosis of recurrence progression bladder are still based on regular but outdated cystoscopy followed by cytological examination. To reduce the number procedures, new reliable biomarkers for predicting tumor behavior must be developed. The aim this study was to confirm our previous results that demonstrated overexpression AP1S1, CDK9, FIGF HDAC11 in muscle-invasive cancer. project management performed using iterative flexible work (Flexible Methodology Innovative Projects Scientific Organizations, FMIPSO). Gene expression analysis genes evaluated 39 newly collected non-invasive tumors. Differential gene calculated ΔΔCt method with GPDH as housekeeping gene. 4,0-fold change used cutoff determine up- or down-regulation compared negative control. Our demonstrate involvement FIGF, CDK9 their potential use candidate characterize invasive phenotype muscle progression, well potentially cystoscopies. We FMIPSO able achieve at optimum level efficiency control all possible constraints scientific organizations.